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Ostarine mk-2866 vs anavar Somatropin is a form of human growth hormone important for the growth of bones and musclesin dogs and humans  . We have previously shown that porphyrin is the most potent inhibitor of LTB4-dependent IGF-1 and LTB4-dependent IGF-binding protein-1 in vitro  . Furthermore, IGF-1 and LTB4-dependent IGF-binding proteins are expressed in the spleen and bone marrow of dogs  . We therefore tested the potential inhibitory effect of porphyrin on LTB4, IGF-1, IGF-binding protein-1, and IGFBP-1 in our experimental model. For this study, we treated adult male dogs with either porphyrin (12 μg ml−1) or estradiol (10 μg ml−1) for 14 days after he was spayed or neutered. After each animal was observed for several hours, we performed two groups of measurements. In the first group, blood samples (5 mL plasma) were collected 24 h after serum samples were collected to assess growth hormone concentration (HGH, GHB); for the second group, serum samples were collected 1 hour after the end of the 14 day treatment period. LTB4 levels were measured in the spleen by ELISA kits (Beckman Coulter Genetix, Franklin Lakes, NJ, USA) using the following kit (QIAGEN, Valencia, CA, USA), with the same reference range as in the study performed on spayed dogs. IGF-1 levels in the spleen were measured by ELISA kits (R&D Systems, Minneapolis, MN, USA), with the same reference range used. IGFBP-1 levels in the spleen were measured by ELISA kits (R&D Systems), using the same reference range used for the study on spayed dogs. Dogs were sacrificed by cervical dislocation 2–3 weeks after hCG treatment was initiated. A single dose of recombinant human chorionic gonadotropin, hCG, 10 μg ml−1, was injected via intrathecal injection needle into the bladder, or by subcutaneous injection at the lower abdomen 1-2 cm below the bladder opening. All animals were sacrificed at the same timepoint; the time of death was recorded by the time of death following hematoma. HGCGs were quantified by commercial ELISA kits (BD Biosciences, San Jose, CA, USA), using the following reference range: 1∶500–1⋅100 ng ml−1. Data are Related Article: